| 產(chǎn)品編號 | bs-1126R |
| 英文名稱 | 5-HT Rabbit pAb |
| 中文名稱 | 5羥色胺抗體 |
| 別 名 | Serotonin; 5-hydroxytryptamine; 3-(2-Aminoethyl)-5-hydroxyindole hydrochloride; 5 hydroxy tryptophan; 5 Hydroxytryptophan; 5HTP; 5-HT, 5-Hydroxytryptamine hydrochloride. 5-羥色胺; 五羥色胺; |
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Specific References (6) | bs-1126R has been referenced in 6 publications.
[IF=5.81] Shengyan Xi. et al. The Ciji-Hua’ai-Baosheng II Formula Attenuates Chemotherapy-Induced Anorexia in Mice With H22 Hepatocellular Carcinoma. Front Pharmacol. 2021; 12: 715824 IHC ; Mouse.
[IF=2.837] Arzu Hizay. et al. Lactobacillus acidophilus regulates abnormal serotonin availability in experimental ulcerative colitis. ANAEROBE. 2023 Apr;80:102710 IHC ; Rat.
[IF=2.43] Yin, Yue, et al. "Tong Xie Yao Fang relieves irritable bowel syndrome in rats via mechanisms involving regulation of 5-hydroxytryptamine and substance P."World Journal of Gastroenterology: WJG 21.15 (2015): 4536. IHC-P ; Rat.
[IF=1.966] Tian L et al. Gingerol inhibits cisplatin-induced acute and delayed emesis in rats and minks by regulating the central and peripheral 5-HT, SP, and DA systems. J Nat Med. 2019 Nov 25. IHC-P ; rat&mink.
[IF=1.918] Haili Zhang. et al. Morphological changes in the digestive tract of the Chinese soft-shelled turtle (Pelodiscus sinensis) during embryonic development. J HISTOTECHNOL. 2022 Aug 01 IHC ; Chicken.
[IF=0.392] Yildiz, M.. et al. The Effect of Chronic Intermittent Cold Exposure on Gastrin-, Somatostatin-, Secretin-, and Serotonin-Containing Cells in the Small Intestine of Rats. Biol Bull+. 2021 Dec;48(3):S95-S106 IHC ; Rat.
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| 產(chǎn)品類型 | 小分子抗體 |
| 研究領(lǐng)域 | 腫瘤 細胞生物 免疫學(xué) 神經(jīng)生物學(xué) 藥物及化合物 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Rat,5-HT |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 0.21268 kDa |
| 檢測分子量 | |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated 5-HT |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Serotonin (pronounced) (5-hydroxytryptamine, or 5-HT) is a monoamine neurotransmitter synthesized in serotonergic neurons in the central nervous system (CNS) and enterochromaffin cells in the gastrointestinal tract of animals including humans. Serotonin is also found in many mushrooms and plants, including fruits and vegetables. Function: Tryptophan is an amino acid occurring naturally in food. In the brain, it is converted to 5-hydroxy-tryptophan (5-HTP), which, in turn, is converted to serotonin. Serotonin is a neurotransmitter essential to regulating appetite, sleep, mood and pain levels. 5－羥色胺在神經(jīng)組織中有重要的功能，目前已肯定中樞神經(jīng)系統(tǒng)有5－羥色胺能神經(jīng)元。5－羥色胺可使大部分交感神經(jīng)節(jié)前神經(jīng)元興奮，而使付交感節(jié)前神經(jīng)元抑制。常用于消化道神經(jīng)內(nèi)分泌細胞及其腫瘤的研究。5-羥色胺，又稱血清素，由消化道EC細胞所分泌。 |
| 產(chǎn)品圖片 |
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-5-HT Polyclonal Antibody, Unconjugated(bs-1126R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-5-HT Polyclonal Antibody, Unconjugated(bs-1126R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
