| 產(chǎn)品編號 | bs-0181R |
| 英文名稱 | Nociceptin receptor Rabbit pAb |
| 中文名稱 | 孤菲肽受體/痛敏肽受體抗體 |
| 別 名 | H.sapiens mRNA for ORL1 receptor; Kappa type 3 opioid receptor; Kappa-type 3 opioid receptor; KOR 3; KOR-3; KOR3; LC132 receptor like; MGC34578; Nociceptin receptor; NOCIR; OOR; Opiate receptor like 1; Opiod receptor like 1; Oprl1; OPRX_HUMAN; ORL1; Orphanin FQ receptor. |
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Specific References (1) | bs-0181R has been referenced in 1 publications.
[IF=2.227] Xingrao Ke. et al. Adverse Maternal Environment Alters Oprl1 Variant Expression in Mouse Hippocampus. ANAT REC. 2022 Aug;: IHC ; Mouse.
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| 研究領(lǐng)域 | 細胞生物 神經(jīng)生物學 信號轉(zhuǎn)導 細胞凋亡 細胞膜受體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human,Mouse,Rat (predicted: Pig,Cow,Dog,GuineaPig,Horse) |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 40 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Nociceptin receptor: 1-47/370 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Nociceptin receptor Receptor for the neuropeptide nocipeptin/orphanin FQ. Has a potential role in modulating a number of brain functions, including instinctive behaviors and emotions. The activity of this receptor is mediated by G proteins which inhibits adenylyl cyclase[subcellular location] integral membrane protein. Belongs to the G-protein coupled receptor 1 family. Function: Receptor for the neuropeptide nociceptin/orphanin FQ. Has a potential role in modulating a number of brain functions, including instinctive behaviors and emotions. The activity of this receptor is mediated by G proteins which inhibits adenylyl cyclase. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: In the brain, isoform KOR3 and isoform KOR3C are most abundant in hypothalamus and periaqueductal gray. Isoform KOR3A is highly expressed in cortex, striatum and brainstem. Isoform KOR3D is highly expressed in cerebellum, hypothalamus and brainstem. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: P41146 Gene ID: 4987 Database links: Entrez Gene: 4987 Human Entrez Gene: 18389 Mouse Omim: 602548 Human SwissProt: P41146 Human SwissProt: P35377 Mouse Unigene: 2859 Human Unigene: 285075 Mouse Unigene: 9762 Rat |
| 產(chǎn)品圖片 |
Sample:
Heart(Rat) lysate at 30ug;
Brain(Rat) lysate at 30ug;
Primary: Anti-Nociceptin receptor (bs-0181R) at 1:200 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution;
Predicted band size : 40kD
Observed band size : 40kD
Sample:
Heart (Mouse) Lysate at 40 ug
Primary: Anti- Nociceptin receptor (bs-0181R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40 kD
Observed band size: 52 kD
免疫組化（IHC）Immunohistochemistry
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Nociceptin receptor Polyclonal Antibody, Unconjugated(bs-0181R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
免疫熒光Immunofluorescence
組織切片：
Tissue/cell: human placenta tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Nociceptin receptor Polyclonal Antibody, Unconjugated(bs-0181R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3conjugated (bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
