| 產(chǎn)品編號 | bs-4188R |
| 英文名稱 | SGPL1 Rabbit pAb |
| 中文名稱 | 磷酸鞘氨醇裂解酶1抗體 |
| 別 名 | FLJ13811; hSPL; KIAA1252; S1P; S1PL; SGPL 1; SGPL1; SGPL1_HUMAN; SP lyase; SP-lyase 1; Sphingosine 1 phosphate aldolase; Sphingosine 1 phosphate lyase 1; Sphingosine-1-phosphate aldolase; Sphingosine-1-phosphate lyase 1; SPL 1; SPL. |
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Specific References (5) | bs-4188R has been referenced in 5 publications.
[IF=11.47] Choi, Won Hoon, et al. "Open-gate mutants of the mammalian proteasome show enhanced ubiquitin-conjugate degradation." Nature Communications 7 (2016). WB ; Human.
[IF=5.853] Mili Minaduola. et al. The circadian clock sets a spatial–temporal window for recent thymic emigrants. IMMUNOL CELL BIOL. 2022 Sep;: IF ; Mouse.
[IF=4.005] Karuppuchamy T et al. Sphingosine-1-Phosphate Lyase Inhibition Alters the S1P Gradient and Ameliorates Crohn's-Like Ileitis by Suppressing Thymocyte Maturation. Inflamm Bowel Dis. 2020 Jan 6;26(2):216-228. IHC-P ; Mouse.
[IF=3.687] Lemos et al. Sphingosine-1-Phosphate Receptor 1 Is Involved in Non-Obese Diabetic Mouse Thymocyte Migration Disorders. (2018) Int.J.Mol.Sci. 19 FC/IHC ; mouse.
[IF=2.25] Zhang X et al. Quercetin ameliorates pulmonary fibrosis by inhibiting SphK1/S1P signaling.Biochem Cell Biol. 2018 Dec;96(6):742-751. WB ; Mouse&Human.
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| 研究領域 | 腫瘤 心血管 免疫學 信號轉導 脂蛋白 新陳代謝 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Dog,Horse) |
| 產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1ug/test,ICC/IF=1:100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 63 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SGPL1: 301-400/568 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Cleaves phosphorylated sphingoid bases (PSBs), such as sphingosine-1-phosphate, into fatty aldehydes and phosphoethanolamine. Elevates stress-induced ceramide production and apoptosis. Function: Cleaves phosphorylated sphingoid bases (PSBs), such as sphingosine-1-phosphate, into fatty aldehydes and phosphoethanolamine. Elevates stress-induced ceramide production and apoptosis. Subcellular Location: Endoplasmic reticulum membrane; Single-pass type III membrane protein. Tissue Specificity: Found in liver and kidney. Similarity: Belongs to the group II decarboxylase family. Sphingosine-1-phosphate lyase subfamily. SWISS: O95470 Gene ID: 8879 Database links: Entrez Gene: 8879 Human Entrez Gene: 20397 Mouse SwissProt: O95470 Human SwissProt: Q8R0X7 Mouse Unigene: 499984 Human Unigene: 412319 Mouse Unigene: 431862 Mouse Unigene: 26953 Rat Omim: 603729 Human
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| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (mouse heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SGPL1) Polyclonal Antibody, Unconjugated (bs-4188R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SGPL1) Polyclonal Antibody, Unconjugated (bs-4188R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SGPL1) Polyclonal Antibody, Unconjugated (bs-4188R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SGPL1) Polyclonal Antibody, Unconjugated (bs-4188R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (SGPL1) polyclonal Antibody, Unconjugated (bs-4188R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Blank control: A549.
Primary Antibody (green line): Rabbit Anti-SGPL1 antibody (bs-4188R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Blank control: A549.
Primary Antibody (green line): Rabbit Anti-SGPL1/FITC Conjugated antibody (bs-4188R-FITC)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG-FITC .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
