| 產(chǎn)品編號 | bs-7056R |
| 英文名稱 | FTO Rabbit pAb |
| 中文名稱 | 脂肪堆積和肥胖相關(guān)蛋白抗體 |
| 別 名 | alpha-ketoglutarate-dependent dioxygenase FTO; Fat mass and obesity-associated protein; FTO_HUMAN; Protein fatso. |
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Specific References (8) | bs-7056R has been referenced in 8 publications.
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| 研究領(lǐng)域 | 心血管 細(xì)胞生物 免疫學(xué) 神經(jīng)生物學(xué) 糖尿病 內(nèi)分泌病 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 58 kDa |
| 檢測分子量 | 58 |
| 細(xì)胞定位 | 細(xì)胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Protein fatso: 165-265/505 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. Has highest activity towards single-stranded RNA containing 3-methyluracil, followed by single-stranded DNA containing 3-methylthymine. Has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine. Has no activity towards 1-methylguanine. Has no detectable activity towards double-stranded DNA. Requires molecular oxygen, alpha-ketoglutarate and iron. Contributes to the regulation of the global metabolic rate, energy expenditure and energy homeostasis. Contributes to the regulation of body size and body fat accumulation. Tissue specificity: Ubiquitously expressed, with relatively high expression in adrenal glands and brain; especially in hypothalamus and pituitary. Function: Dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. Has highest activity towards single-stranded RNA containing 3-methyluracil, followed by single-stranded DNA containing 3-methylthymine. Has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine. Has no activity towards 1-methylguanine. Has no detectable activity towards double-stranded DNA. Requires molecular oxygen, alpha-ketoglutarate and iron. Contributes to the regulation of the global metabolic rate, energy expenditure and energy homeostasis. Contributes to the regulation of body size and body fat accumulation. Subunit: Monomer. May also exist as homodimer. Subcellular Location: Nucleus. Tissue Specificity: Ubiquitously expressed, with relatively high expression in adrenal glands and brain; especially in hypothalamus and pituitary. DISEASE: Defects in FTO are the cause of growth retardation developmental delay coarse facies and early death (GDFD) [MIM:612938]. A severe polymalformation syndrome characterized by postnatal growth retardation, microcephaly, severe psychomotor delay, functional brain deficits and characteristic facial dysmorphism. In some patients, structural brain malformations, cardiac defects, genital anomalies, and cleft palate are observed. Early death occurs by the age of 3 years. Similarity: Belongs to the fto family. SWISS: Q9C0B1 Gene ID: 79068 Database links: Entrez Gene: 79068?Human Entrez Gene: 26383?Mouse Omim: 610966?Human SwissProt: Q9C0B1?Human SwissProt: Q8BGW1?Mouse SwissProt: Q2A121?Rat |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Mouse Adrenal gland tissue lysates
Lane 2: Rat Adrenal gland tissue lysates
Lane 3: Human SH-SY5Y cell lysates
Lane 4: Human HeLa cell lysates
Lane 5: Human U87MG cell lysates
Lane 6: Human MOLT4 cell lysates
Primary: Anti-FTO (bs-7056R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58 kDa
Observed band size: 60 kDa
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