| 產(chǎn)品編號 | bs-6765R |
| 英文名稱 | Perilipin A+B Rabbit pAb |
| 中文名稱 | 脂滴包被蛋白A+B抗體 |
| 別 名 | Lipid droplet associated protein; PERIA; PERIB; Perilipin A; Perilipin; Perilipin B; PerilipinA; PerilipinB; PLIN. |
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Specific References (2) | bs-6765R has been referenced in 2 publications.
[IF=5.37] James, Sally, et al. "Multiparameter Analysis of Human Bone Marrow Stromal Cells Identifies Distinct Immunomodulatory and Differentiation-Competent Subtypes." Stem cell reports 4.6 (2015): 1004-1015. Human.
[IF=4.357] Li et al. Morusin suppresses breast cancer cell growth in vitro and in vivo through C/EBPβ and PPARγ mediated lipoapoptosis. (2015) J.Exp.Clin.Cancer.Res. 34:137 WB ; Human.
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| 研究領(lǐng)域 | 腫瘤 心血管 細胞生物 信號轉(zhuǎn)導 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Human,Mouse (predicted: Sheep,Chicken,Dog) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=0.2ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 54-56 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Perilipin A+B: Mixed peptides |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Perilipin is an intracellular neutral lipid droplet protein that is hormonally regulated. This protein is localized exclusively to the surface of lipid droplets. In response to lypotic stimuli, perilipin is phosphorylated by protein kinase A. Once activated, perilipin has inhibitory affects upon hormone-sensitive lipase (HSL), a protein that mediates the hydrolysis of triacylglycerol, the major form of stored energy in the body. Perilipin expression is limited to adipocytes and steroidogenic cells. There are currently two known isoforms, Perilipin A and B. Both of these proteins are encoded by a single-copy gene and are the result of differential splicing events. Function: Perilipin A: Modulator of adipocyte lipid metabolism. Coats lipid storage droplets to protect them from breakdown by hormone-sensitive lipase (HSL). Its absence may result in leanness. Perilipin B: May be involved in development and maintenance of adipose tissue. Subunit: Perilipin A: Interacts with ABHD5. Subcellular Location: Perilipin A: Lipid droplet. Note=Lipid droplet surface-associated. Perilipin B: Membrane; Peripheral membrane protein. Tissue Specificity: Perilipin A: Adipocytes. Perilipin B: Milk lipid globules. Post-translational modifications: Perilipin A: Major cAMP-dependent protein kinase-substrate in adipocytes, also dephosphorylated by PP1. When phosphorylated, may be maximally sensitive to HSL and when unphosphorylated, may play a role in the inhibition of lipolysis, by acting as a barrier in lipid droplet. Perilipin B: Acylated; primarily with C14, C16 and C18 fatty acids. DISEASE: Perilipin A: Defects in PLIN1 are the cause of familial partial lipodystrophy type 4 (FPLD4) [MIM:613877]. FPLD4 is a form of lipodystrophy characterized by loss of subcutaneous adipose tissue primarily affecting the lower limbs, insulin-resistant diabetes mellitus, hypertriglyceridemia, and hypertension. Perilipin B: Similarity: Perilipin A: Belongs to the perilipin family. Perilipin B: Belongs to the perilipin family. SWISS: O60240 Gene ID: 5346 Database links: Entrez Gene: 5346 Human Entrez Gene: 103968 Mouse Omim: 170290 Human SwissProt: O60240 Human SwissProt: Q8CGN5 Mouse Unigene: 103253 Human |
| 產(chǎn)品圖片 |
Sample: HepG2 Cell (Human) Lysate at 30 ug
Primary: Anti-Perilipin A+B (bs-6765R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54-56kD
Observed band size: 50kD
Sample:
Stomach (Mouse) Lysate at 40 ug
Primary: Anti-Perilipin A+B? (bs-6765R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54-56 kD
Observed band size: 56 kD
This image was generously provided by James Fox, PhD. at the University of York. Immunostaining of perilipin-positive adipocytes (red) using Rabbit Anti-Perilipin A+B Polyclonal Antibody (bs-6765R) in bone marrow of IL-7cre Rosa26-EYFP mice with DAPI (blue) nuclear counterstain.
Blank control: U937 (fixed with 2% paraformaldehyde (10 min)). Primary Antibody:Rabbit Anti- Perilipin A+B antibody(bs-6765R), Dilution: 0.2μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Blank control: MCF7(blue). Primary Antibody: Rabbit Anti-Perilipin A+B/AF647 Conjugated antibody (bs-6765R-AF647), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
