| 產(chǎn)品編號 | bs-17574R |
| 英文名稱 | OXA1L Rabbit pAb |
| 中文名稱 | OXA1L蛋白抗體 |
| 別 名 | HSA; Mitochondrial inner membrane protein OXA1L; OXA1; OXA1 like protein; OXA1-like protein; OXA1Hs; Oxa1l; OXA1L_HUMAN; Oxidase(cytochrome c) assembly 1 like; Oxidase assembly 1 like protein; Oxidase assembly 1-like protein. |
| 研究領(lǐng)域 | 細胞生物 信號轉(zhuǎn)導(dǎo) 細胞類型標志物 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat,Rabbit) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 49 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human OXA1L: 301-400/435 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Required for the insertion of integral membrane proteins into the mitochondrial inner membrane. Essential for the activity and assembly of cytochrome oxidase. Required for the correct biogenesis of ATP synthase and complex I in mitochondria. Subcellular Location: Mitochondrion inner membrane. Similarity: Belongs to the OXA1/ALB3/YidC family. SWISS: Q15070 Gene ID: 5018 Database links: Entrez Gene: 100719154 Guinea pig Entrez Gene: 100056354 Horse Entrez Gene: 5018 Human Entrez Gene: 69089 Mouse Entrez Gene: 562963 Zebrafish Omim: 601066 Human SwissProt: Q15070 Human SwissProt: Q8BGA9 Mouse Unigene: 151134 Human Unigene: 182340 Mouse |
| 產(chǎn)品圖片 |
Sample:
Hcclm3(Human) Cell Lysate at 30 ug
Primary: Anti-OXA1L (bs-17574R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 49 kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (OXA1L) Polyclonal Antibody, Unconjugated (bs-17574R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
