| 產(chǎn)品編號(hào) | bsm-33189M |
| 英文名稱 | ECFP Mouse mAb |
| 中文名稱 | 增強(qiáng)型青色熒光蛋白單克隆抗體 |
| 別 名 | Cyan Fluorescent Protein; Enhanced Cyan Fluorescent Protein. |
| 產(chǎn)品類型 | 標(biāo)簽抗體 |
| 研究領(lǐng)域 | 細(xì)胞生物 |
| 抗體來(lái)源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號(hào) | 10H5 |
| 交叉反應(yīng) | Recombinant protein |
| 產(chǎn)品應(yīng)用 | WB=1:1000-5000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | kDa |
| 檢測(cè)分子量 | |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant ECFP |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Cyan Fluorescent Protein (CFP) is a genetic mutant of green fluorescent protein (GFP) originally derived from the jellyfish Aequorea victoria. The fluorescence of EYFP was detected with an excitation filter of 485 nm and an emission filter of 545 nm, and that of ECFP was detected with filters of 420 and 486 nm (Perkin Elmer), respectively. In the present reporter assay, EYFP and ECFP were selected as a reporter protein and an internal control for normalization of transfection, respectively.
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| 產(chǎn)品圖片 |
Sample:
Lane 1: ECFP-Tagged fusion protein (full length) Overexpression E.coli Lysate (Cat#: bs-33189P) at 4 ug
Primary: Anti-ECFP-Tag (bsm-33189M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 27 kD
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