| 產(chǎn)品編號 | bs-22732R |
| 英文名稱 | CD4 Rabbit pAb |
| 中文名稱 | CD4抗體 |
| 別 名 | CD4(L3T4); CD4 antigen(p55); CD4 Antigen; CD4 molecule; CD4 Receptor; CD4+Lymphocyte deficiency, included; CD4mut; L3T4; Leu3; Ly-4; Lymphocyte antigen CD4; MGC165891; p55; T Cell Antigen T4; T cell antigen T4/LEU3; T cell differentiation antigen L3T4; T cell OKT4 deficiency, included; T cell surface antigen T4/Leu 3; T cell surface antigen T4/Leu3; T Cell Surface Glycoprotein CD4; W3/25; W3/25 antigen; T-cell surface glycoprotein CD4 isoform 1 precursor; CD4_HUMAN. |
| 研究領(lǐng)域 | 腫瘤 細胞生物 免疫學(xué) 干細胞 轉(zhuǎn)錄調(diào)節(jié)因子 細胞表面分子 t-淋巴細胞 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Pig,Sheep,Cow,Dog,GuineaPig,MACFA) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 48 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CD4: 385-457/457 <Cytoplasmic> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010]. Function: Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. Subunit : Associates with LCK. Binds to HIV-1 gp120 and to P4HB/PDI and upon HIV-1 binding to the cell membrane, is part of P4HB/PDI-CD4-CXCR4-gp120 complex. Interacts with HIV-1 Envelope polyprotein gp160 and protein Vpu. Interacts with Human Herpes virus 7 capsid proteins. Interacts with PTK2/FAK1; this interaction requires the presence of HIV-1 gp120. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Note=Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum. Post-translational modifications: Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts. Similarity: Contains 3 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 Ig-like V-type (immunoglobulin-like) domain. SWISS: P01730 Gene ID: 920 Database links: Entrez Gene: 920 Human Entrez Gene: 12504 Mouse Omim: 186940 Human SwissProt: P06332 Mouse SwissProt: P01730 Human Unigene: 631659 Human Unigene: 2209 Mouse 此抗體可識別55KDⅠ型單鏈穿膜糖蛋白。 CD4分子是存在于大多數(shù)輔助/誘導(dǎo)T細胞表面的59kDa的糖蛋白。正常淋巴組織中CD4的表達數(shù)量多于CD8，此抗體主要用于標記輔助/誘導(dǎo)T細胞，與CD8單抗聯(lián)合使用對外周血淋巴細胞分型。 CD4抗原是HLA-II類分子和人類免疫缺陷病毒(HIV)-愛滋病的受體，在35-50%外周血淋巴細胞-輔助和誘導(dǎo)T細胞(Th/Ti)和70-80%人胸腺細胞上表達，在人的單核細胞表面也有低密度的表達。 CD4抗原有膜結(jié)合型和可溶性兩種形式。Th/Ti可輔助Ig產(chǎn)生和T細胞毒T細胞的作用。 |
| 產(chǎn)品圖片 |
Sample:
Spleen (Mouse) Lysate at 40 ug
Lymph node (Mouse) Lysate at 40 ug
Thymus (Mouse) Lysate at 40 ug
Cerebrum (Mouse) Lysate at 40 ug
Thymus (Rat) Lysate at 40 ug
Molt-4(Human) Cell Lysate at 30 ug
HL60(Human) Cell Lysate at 30 ug
Jurkat(Human) Cell Lysate at 30 ug
Raji(Human) Cell Lysate at 30 ug
Primary: Anti- CD4 (bs-22732R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 53 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
