| 產(chǎn)品編號 | bs-0891R-AP |
| 英文名稱 | Rabbit Anti-Pokemon/ZBTB7A/AP Conjugated antibody |
| 中文名稱 | 堿性磷酸酶（AP）標(biāo)記的撲克蒙蛋白抗體 |
| 別 名 | Factor binding IST protein 1; Factor that binds to inducer of short transcripts protein 1; FBI-1; FBI1; HIV-1 1st-binding protein 1; Leukemia/lymphoma related factor; LRF; Pokemon; TIP21; TTF-I interacting peptide 21; ZBTB7; ZBTB7A; Zinc finger and BTB domain-containing protein 7A; ZBT7A_HUMAN. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領(lǐng)域 | 腫瘤 免疫學(xué) 微生物學(xué) 轉(zhuǎn)錄調(diào)節(jié)因子 細(xì)菌及病毒 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human, (predicted: Mouse, Rat, ) |
| 產(chǎn)品應(yīng)用 | WB=1:50-200 IHC-P=1:50-200 IHC-F=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 63kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Pokemon |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: Pokemon, the POK erythroid myeloid ontogenic factor, not only regulates the expression of many genes, but also plays an important role in cell tumorigenesis. To investigate the molecular mechanism regulating expression of the Pokemon gene in humans, its 5'-upstream region was cloned and analyzed. Transient analysis revealed that the Pokemon promoter is constitutive. Deletion analysis and a DNA decoy assay indicated that the NEG-U and NEG-D elements were involved in negative regulation of the Pokemon promoter, whereas the POS-D element was mainly responsible for its strong activity. Electrophoretic mobility shift assays suggested that the NEG-U, NEG-D and POS-D elements were specifically bound by the nuclear extract from A549 cells in vitro. Mutation analysis demonstrated that cooperation of the NEG-U and NEG-D elements led to negative regulation of the Pokemon promoter. Moreover, the NEG-U and NEG-D elements needed to be an appropriate distance apart in the Pokemon promoter in order to cooperate. Taken together, our results elucidate the mechanism underlying the regulation of Pokemon gene transcription, and also define a novel regulatory sequence that may be used to decrease expression of the Pokemon gene in cancer gene therapy. Function: Plays a key role in the instruction of early lymphoid progenitors to develop into B lineage by repressing T-cell instructive Notch signals (By similarity). Specifically represses the transcription of the CDKN2A gene. Efficiently abrogates E2F1-dependent CDKN2A transactivation/de-repression. Binds to the consensus sequence 5'-[GA][CA]GACCCCCCCCC-3'. Subunit: Interacts with BCL6. Subcellular Location: Nucleus. Tissue Specificity: Widely expressed. In normal thymus, expressed in medullary epithelial cells and Hassle's corpuscles (at protein level). In tonsil, expressed in squamous epithelium and germinal center lymphocytes (at protein level). Up-regulated in a subset of lymphomas, as well as in a subset of breast, lung, colon, prostate and bladder carcinomas (at protein level). Similarity: Contains 1 BTB (POZ) domain. Contains 4 C2H2-type zinc fingers. Database links: Entrez Gene: 51341 Human Entrez Gene: 16969 Mouse Omim: 605878 Human SwissProt: O95365 Human SwissProt: O88939 Mouse Unigene: 591384 Human Unigene: 20920 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. Pokemon(7APOK Erythroid Myeloid Ontogenic factor)能夠控制將正常細(xì)胞變成癌細(xì)胞所需的途徑,Pokemon蛋白是轉(zhuǎn)錄因子蛋白家族的一員，并且在人類癌癥中發(fā)生了突變。另外Pokemon蛋白作為轉(zhuǎn)錄因子參與一些細(xì)胞基因轉(zhuǎn)錄的調(diào)節(jié),并在細(xì)胞分化過程中發(fā)揮著關(guān)鍵、多效性的功能。近來發(fā)現(xiàn),Poke-mon在致癌轉(zhuǎn)化過程中發(fā)揮著至關(guān)重要的功能,并與腫瘤的發(fā)生密切相關(guān)。 這種蛋白很可能在實質(zhì)腫瘤中處于重要地位,還有學(xué)者認(rèn)為：Pokemon在一定類型的B細(xì)胞和T細(xì)胞淋巴瘤中水平非常高，并發(fā)現(xiàn)具有高水平Pokemon蛋白表達(dá)對腫瘤惡性程度改變更大。 主要定位于細(xì)胞核。 撲克蒙”蛋白又稱“波克曼”蛋白。 |
