| 產(chǎn)品編號 | bs-2683R-FITC |
| 英文名稱 | Rabbit Anti-CLEC2D/FITC Conjugated antibody |
| 中文名稱 | FITC標記的CLEC2D蛋白抗體 |
| 別 名 | C-type lectin domain family 2 member D; C type lectin domain family 2 member D isoform 5; CLAX; LLT1; OCIL; Lectin like NK cell receptor; Lectin like transcript 1; Osteoclast inhibitory lectin; CLC2D_HUMAN; CLEC2D; CLEC2D protein; Lectin-like NK cell receptor; Lectin-like transcript 1; LLT-1; Osteoclast inhibitory lectin. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領域 | 免疫學 細胞表面分子 t-淋巴細胞 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse, (predicted: Human, Rat, Dog, Pig, Horse, Rabbit, ) |
| 產(chǎn)品應用 | Flow-Cyt=2ug/Test IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 10kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CLEC2D |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: This gene encodes a member of the natural killer cell receptor C-type lectin family. The encoded protein inhibits osteoclast formation and contains a transmembrane domain near the N-terminus as well as the C-type lectin-like extracellular domain. Several alternatively spliced transcript variants have been identified, but the full-length nature of every transcript has not been defined. Function: Receptor for KLRB1 that protects target cells against natural killer cell-mediated lysis. Inhibits osteoclast formation. Inhibits bone resorption. Modulates the release of interferon-gamma. Binds high molecular weight sulfated glycosaminoglycans. Subunit: Homodimer or heterodimer; disulfide-linked. Subcellular Location: Cell membrane; Single-pass type II membrane protein. Cell surface. Isoform 2, 4: Endoplasmic reticulum. Tissue Specificity: Detected in fetal heart, brain, lung, chondrocytes, perichondrium and osteoblasts, and in adult splenocytes, thymocytes, lymph-node cells, osteoblasts, growth plate chondrocytes and skeltal muscle overlying the bone (at protein level). Ubiquitous. Detected in thymus, bone marrow, lung, gut, heart, skeletal muscle, ovary, spleen, ileum, liver and kidney. Similarity: Contains 1 C-type lectin domain. Database links: Entrez Gene: 29121 Human Entrez Gene: 93694 Mouse Omim: 605659 Human SwissProt: Q9UHP7 Human SwissProt: Q91V08 Mouse Unigene: 268326 Human Unigene: 197536 Mouse Unigene: 22573 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產(chǎn)品圖片 |
Blank control (blue line): Raw264.7 (blue).
Primary Antibody (red line): Rabbit Anti-CLEC2D antibody (bs-2683R-FITC)in Overexpression hCLEC2D Raw264.7 Dilution: 1μg /10^6 cells
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature.Acquisition of 20,000 events was performed.
Tongji University/Professor Jia’s laboratory
Blank control:Mouse spleen.
Primary Antibody (green line): Rabbit Anti-CLEC2D antibody (bs-2683R-FITC)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
