| 產(chǎn)品編號 | bs-12279R-BF647 |
| 英文名稱 | Rabbit Anti-SOHLH2/BF647 Conjugated antibody |
| 中文名稱 | BF647標記的精子卵子結(jié)合生成堿性螺旋蛋白2抗體 |
| 別 名 | FLJ20449; FLJ57222; Solh2; Spermatogenesis- and oogenesis-specific basic helix-loop-helix-containing protein 2; TEB1; SOLH2_HUMAN. |
| 規(guī)格價格 | 100ul/2980元 購買 大包裝/詢價 |
| 說 明 書 | 100ul |
| 研究領(lǐng)域 | 細胞生物 發(fā)育生物學(xué) 干細胞 轉(zhuǎn)錄調(diào)節(jié)因子 表觀遺傳學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | (predicted: Human, Mouse, Rat, ) |
| 產(chǎn)品應(yīng)用 | ICC=1:50-200 IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 47kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from Human SOHLH2 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 儲 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 產(chǎn)品介紹 |
background: SOHLH2 is a 425 amino acid nuclear protein that contains one basic helix-loop-helix (bHLH) domain through which it may function as a transcription factor during oogenesis and spermatogenesis. SOHLH2 exists as two alternatively spliced isoforms that are encoded by a gene which maps to human chromosome 13q13.3. Chromosome 13 houses over 400 genes, such as BRCA2 and RB1, and comprises nearly 4% of the human genome. Trisomy 13, also known as Patau syndrome, is deadly and the few who survive past one year suffer from permanent neurologic defects, difficulty eating and vulnerability to serious respiratory infections. Function: Sohlh2, spermatogenesis- and oogenesis-specific basic helix-loop-helix-containing protein 2, is a basic loop helix transcription factor. Sohlh2 is normally expressed in pre-meiotic germ cells and testis and involved in spermatogenesis and oogenesis. Male Sohlh2 deficient mice are infertile due to a block in spermatogenesis. Subcellular Location: Nuclear Similarity: Contains 1 bHLH (basic helix-loop-helix) domain. Database links: UniProtKB/Swiss-Prot: Q9NX45.2 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
