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F-Actin Rabbit pAb (bs-1571R)

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50ul/1180.00元

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產(chǎn)品編號	bs-1571R
英文名稱	F-Actin Rabbit pAb
中文名稱	纖維狀肌動蛋白抗體
別名	ACTB_HUMAN; Actin, cytoplasmic 1; ACTB; EC:3.6.4.- ; Beta actin; Actin, cytoplasmic 1, N-terminally processed; actin beta; BNS; CSMH; DDS1; THC8; BKRNS; BRWS1; PS1TP5BP1; Actin cytoplasmic 1; β actin; β-actin; βactin;
	Specific References (31) \| bs-1571R has been referenced in 31 publications. [IF=6.126] Taketo Suzuki. et al. Vascular Shutdown by Photodynamic Therapy Using Talaporfin Sodium. Cancers. 2020 Sep;12(9):2369 ICC ; Human. PubMed:32825648 [IF=5.79] Yang et al. Rab5-mediated VE-cadherin internalization regulates the barrier function of the lung microvascular endothelium. (2015) Cell.Mol.Life.Sci. 72:4849-66 WB,ICC ; Human. PubMed:26112597 [IF=5.391] Yagi H et al. Gα13-mediated LATS1 down-regulation contributes to epithelial-mesenchymal transition in ovarian cancer. FASEB J. 2019 Sep 28:fj201901278R. ICF ; Human. PubMed:31569999 [IF=4.556] Tomoko Okada. et al. Scanning Electron-Assisted Dielectric Microscopy Reveals Autophagosome Formation by LC3 and ATG12 in Cultured Mammalian Cells. Int J Mol Sci. 2021 Jan;22(4):1834 IF ; Rat. PubMed:33673233 [IF=4.421] Bareera Qamar. et al. Intracellular Delivery of Active Proteins by Polyphosphazene Polymers. Pharmaceutics. 2021 Feb;13(2):249 ICC ; Human. PubMed:33578893 [IF=4.011] Shiozawa M et al. Biological reaction control using topography regulation of nanostructured titanium. Sci Rep. 2020 Feb 12;10(1):2438. ICF ; Rat. PubMed:32051472 [IF=3.73] Zhong, Weiliang, et al. "Regulation of Fibrochondrogenesis of Mesenchymal Stem Cells in an Integrated Microfluidic Platform Embedded with Biomimetic Nanofibrous Scaffolds." PLOS ONE 8.4 (2013): e61283. Rat. PubMed:23637803 [IF=3.73] Su, Bao, et al. "In Vitro and In Vivo Evaluations of Nano-Hydroxyapatite/Polyamide 66/Glass Fibre (n-HA/PA66/GF) as a Novel Bioactive Bone Screw." PLOS ONE 8.7 (2013): e68342. Mouse. PubMed:23861888 [IF=3.492] Tran X. N. Huy. et al. Cobalt (II) Chloride Regulates the Invasion and Survival of Brucella abortus 544 in RAW 264.7 Cells and B6 Mice. PATHOGENS. 2022 May;11(5):596 WB ; Mouse. PubMed:35631117 [IF=3.22] Zhong, Weiliang, et al. "An integrated microfluidic device for characterizing chondrocyte metabolism in response to distinct levels of fluid flow stimulus."Microfluidics and Nanofluidics: 1-11. PubMed:10.1007/s10404-013-1186-9 [IF=2.8] Zhai J et al. An increase of estrogen receptor α protein level regulates BDE-209-mediated blood-testis barrier disruption during spermatogenesis in F1 mice. (2018) Environ Sci Pollut Res Int. WB,IHF&ICF ; Mouse. PubMed:30565106 [IF=2.8] Kang, Hongyan, et al. "A Potential Gravity-Sensing Role of Vascular Smooth Muscle Cell Glycocalyx in Altered Gravitational Stimulation." Astrobiology (2013). WB ; Rat. PubMed:23848471 [IF=2.788] Alisha Wehdnesday Bernard Reyes. et al. Formyl peptide receptor 2 (FPR2) antagonism is a potential target for the prevention of Brucella abortus 544 infection. Immunobiology. 2021 May;226:152073 WB ; Mouse. PubMed:33657463 [IF=2.78] Jing et al. Expression of immunoglobulin G in human podocytes, and its role in cell viability and adhesion. (2018) Int.J.Mol.Med. 41:3296-3306 IF ; Human. PubMed:29512722 [IF=2.766] Okada T et al. Nanoscale imaging of the adhesion core including integrin β1 on intact living cells using scanning electron-assisted dielectric-impedance microscopy. (2018) PLOS ONE, 13(9), e0204133. IF，ICC ; Mouse. PubMed:30235285 [IF=2.766] Bwalya et al. Pentosan polysulfate inhibits IL-1β-induced iNOS, c-Jun and HIF-1α upregulation in canine articular chondrocytes. (2017) PLoS.One. 12:e0177144 WB ; Dog. PubMed:28472120 [IF=2.74] Clark, Nicolette M., et al. "The human, F-actin-based cytoskeleton as a mutagen sensor." Cancer Cell International 17.1 (2017): 121. IHC-P ; Human. PubMed:29255378 [IF=2.74] Clark et al. The human, F-actin-based cytoskeleton as a mutagen sensor. (2017) Cancer.Cell.Int. 17:121 IF(IHC-P) ; Human. PubMed:29255378 [IF=2.559] Li X et al. LncRNA FTX inhibits hippocampal neuron apoptosis by regulating miR-21-5p/SOX7 axis in a rat model of temporal lobe epilepsy.Biochem Biophys Res Commun.?2019 Apr 23;512(1):79-86. WB ; Rat. PubMed:30871773 [IF=2.55] Zhao, Ran, et al. "Hepatoma cell line HepG2. 2.15 demonstrates distinct biological features compared with parental HepG2." World journal of gastroenterology: WJG 17.9 (2011): 1152. WB ; Human. PubMed:21448419 [IF=2.52] Chen, S. M., et al. "Cofilin is correlated with sperm quality and influences sperm fertilizing capacity in humans." Andrology (2016). WB ; Human. PubMed:27369112 [IF=2.22] Li, Haihong, et al. "Human eccrine sweat gland cells reconstitute polarized spheroids when subcutaneously implanted with Matrigel in nude mice." Journal of molecular histology 47.5 (2016): 485-490. IHC-P ; Mouse. PubMed:27492422 [IF=1.86] Siar, Chong Huat, et al. "Invadopodia proteins, cortactin, N‐WASP and WIP differentially promote local invasiveness in ameloblastoma." Journal of Oral Pathology & Medicine (2016). IHC-P ; Human. PubMed:26752341 [IF=1.84] Zhang, Pengfei, et al. "Proteome analysis of egg yolk after exposure to zinc oxide nanoparticles." Theriogenology (2017). WB ; Chicken. PubMed:28460670 [IF=1.69] Guo, Xiliang, and Xuezheng Liu. "Nogo receptor knockdown and ciliary neurotrophic factor attenuate diabetic retinopathy in streptozotocin-induced diabetic rats." Molecular Medicine Reports. (2017). IHC-P ; Rat. PubMed:28656312 [IF=0.88] Murakami et al. Roles of Ras Homolog A in Invasive Ductal Breast Carcinoma. (2016) Acta.Histochem.Cytochem. 49:131-140 IHC ; Human. PubMed:27917007 [IF=0.86] Xie, Chaoming, Xiong Lu, and Kefeng Wang. "Pulse Electrochemical Synthesis of Spherical Hydroxyapatite and Silver Nanoparticles Mediated by the Polymerization of Polypyrrole on Metallic Implants for Biomedical Applications." Particle & Particle Systems Characterization (2015). Rat. PubMed:10.1002/ppsc.201400245 [IF=0.18] Du, Ailin, et al. "Damage of hippocampal neurons in rats with chronic alcoholism." Neural regeneration research 9.17 (2014): 1610. IHC-P ; Rat. PubMed:25368648 [IF=0] Okada T et al. High-resolution imaging of autophagosome formation by LC3 and ATG12 in cells using scanning electron-assisted dielectric microscopy. Research Square; 2020 Feb 7. ICF ; Mouse&Rat. PubMed:doi:10.21203/rs.2.22874/v1 [IF=0] Bwalya E et al. Pentosan Polysulfate Sodium Restores the Phenotype of Dedifferentiated Monolayer Canine Articular Chondrocytes Cultured in Alginate Bea. J Tissue Sci Eng 2018, 9:1. WB ; Dog. PubMed:DOI:10.4172/2157-7552.1000218
研究領(lǐng)域	免疫學細胞骨架
抗體來源	Rabbit
克隆類型	Polyclonal
克隆號
交叉反應	Human,Mouse,Rat (predicted: Pig,Sheep,Cow)
產(chǎn)品應用	WB=1:1000-5000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=3μg /test not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
理論分子量	42 kDa
檢測分子量
細胞定位	細胞漿
性狀	Liquid
濃度	1mg/ml
免疫原	Synthetic MAP peptide derived from human beta-Actin: 1-50/374
亞型	IgG
純化方法	affinity purified by Protein A
緩沖液	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
產(chǎn)品介紹	In vertebrates, three main groups of actin isoforms, alpha, beta, and gamma have been identified. The alpha actins, found in muscletissues, are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Individual subunits of microfilaments are known as globular actin (G-actin). G-actin subunits assemble into long filamentous polymers called F-actin. SWISS: P60709 Gene ID: 60 Database links: Entrez Gene: 60 Human Entrez Gene: 11461 Mouse Entrez Gene: 100009272 Rabbit Entrez Gene: 81822 Rat Omim: 102630 Human SwissProt: P60709 Human SwissProt: P60710 Mouse SwissProt: P29751 Rabbit SwissProt: P60711 Rat Unigene: 520640 Human Unigene: 708120 Human Unigene: 727576 Human Unigene: 328431 Mouse Unigene: 391967 Mouse Unigene: 94978 Rat 纖維狀肌動蛋白F-Actin主要存在于橫紋肌原肌纖維細絲及平滑肌中，是肌肉運動和細胞運動的重要蛋白。
產(chǎn)品圖片	Sample: Lane1:Hela (Human) Whole Cell Lysate at 30 ug Lane2:Jurkat (Human) Whole Cell Lysate at 30 ug Lane3:293T (Human) Whole Cell Lysate at 30 ug Lane4:Hepg2 (Human) Whole Cell Lysate at 30 ug Lane5:A431 (Human) Whole Cell Lysate at 30 ug Lane6:A549 (Human) Whole Cell Lysate at 30 ug Lane7:H9C2 (Rat) Whole Cell Lysate at 30 ug Lane8:Testis (Mouse) Tissue Lysate at 30 ug Primary: Anti-F-Actin(bs-1571R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 42 kD Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (F-Actin) Polyclonal Antibody, Unconjugated (bs-1571R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (F-Actin) Polyclonal Antibody, Unconjugated (bs-1571R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Blank control (blue line): Hela (blue). Primary Antibody (green line): Rabbit Anti- F-Actin antibody (bs-1571R) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 80% methanol (5 min at -20℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature . Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

	免疫組化常用試劑
	免疫印跡常用試劑
	各種標記二抗

	1、抗體溶解方法
	2、抗體修復方式
	3、常用試劑的配制
	4、免疫組化操作步驟
	5、免疫組化問題解答
	6、Western Blotting 操作步驟
	7、Western Blotting 問題解答
	8、關(guān)于肽鏈的設計
	9、多肽的溶解與保存
	10、酶標抗體效價測定程序

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