| 產(chǎn)品編號 | bs-1185R |
| 英文名稱 | TRAF3 Rabbit pAb |
| 中文名稱 | 腫瘤壞死因子受體相關(guān)蛋白3抗體 |
| 別 名 | CAP 1; CAP1; CD40 associated protein 1; CD40 binding protein; CD40 bp; CD40 receptor associated factor 1; CD40bp; CRAF 1; CRAF1; LAP 1; LAP1; LMP1; Trafamn; LMP1 associated protein; MIPT3; TNF receptor associated factor 3; TRAF 3; TRAF3_HUMAN. |
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Specific References (2) | bs-1185R has been referenced in 2 publications.
[IF=8.025] Wanwan Zhang. et al. Nectin4 antagonises type I interferon production by targeting TRAF3 for autophagic degradation and disrupting TRAF3-TBK1 complex formation. INT J BIOL MACROMOL. 2022 Oct;218:654 WB ; Human.
[IF=3.212] Yi Zhuet al. MicroRNA MiR-27a-5p Alleviates the Cerulein-Induced Cell Apoptosis and Inflammatory Injury of AR42J Cells by Targeting Traf3 in Acute Pancreatitis. Inflammation
. 2020 Oct;43(5):1988-1998. WB ; rat.
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| 研究領(lǐng)域 | 腫瘤 信號轉(zhuǎn)導(dǎo) 細胞凋亡 細胞膜受體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1μg/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 62 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TRAF3: 101-200/568 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the signal transduction from, members of the TNF receptor (TNFR) superfamily. This protein participates in the signal transduction of CD40, a TNFR family member important for the activation of the immune response. This protein is found to be a critical component of the lymphotoxin-beta receptor (LTbetaR) signaling complex, which induces NF-kappaB activation and cell death initiated by LTbeta ligation. Epstein-Barr virus encoded latent infection membrane protein-1 (LMP1) can interact with this and several other members of the TRAF family, which may be essential for the oncogenic effects of LMP1. Three alternatively spliced transcript variants encoding two distinct isoforms have been reported. [provided by RefSeq]. Function: Adapter protein and signal transducer that links members of the tumor necrosis factor receptor family to different signaling pathways by association with the receptor cytoplasmic domain and kinases. Seems to be involved in activation of NF-kappa-B and JNK and in apoptosis. Is regulated by TANK/ITRAF which competes with TNFRSF5/CD40 for binding. Seems to play a role T-cell dependent immune responses (By similarity). Subunit: "Homotrimer (Probable). Heteromer with TRAF5. Binds to TNFRSF5/CD40. Associates with LTBR/TNFRSF3, TNFRSF4, TNFRSF8/CD30, TNFRSF17/BCMA and EDAR, MAP3K5, MAP3K14, and TRAF-interacting protein TRIP and TRAF and TNF receptor associated protein TTRAP. Binds to TANK/ITRAF and TRAF3IP1. Interacts with TICAM1. Interacts with OTUD5. Tissue Specificity: In adult, highest in brain. Also found in kidney, heart, thymus, spleen, lung, muscle, testis and ovary. Not found in liver. Similarity: Contains 1 MATH domain. Contains 1 RING-type zinc finger. Contains 2 TRAF-type zinc fingers. SWISS: Q13114 Gene ID: 7187 Database links: Entrez Gene: 7187 Human Entrez Gene: 22031 Mouse Omim: 601896 Human SwissProt: Q13114 Human SwissProt: Q60803 Mouse Unigene: 510528 Human Unigene: 27431 Mouse Unigene: 12033 Rat TRAF3是作用最為廣泛的一種TRAF蛋白，它可以與LTb R、CD40、TNFRII和LMP1結(jié)合，并介導(dǎo)CD30、LMP1活化NF-kB。TRAF3與CD40結(jié)合后可以挽救凋亡的B細胞， TRAF3還參與CD40在B細胞中上調(diào)CD23的作用。 |
| 產(chǎn)品圖片 |
Sample:Spleen(Mouse)Lysate at 40 ug
Primary: Anti-TRAF3(bs-1185R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62 kD
Observed band size: 62 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TRAF3) Polyclonal Antibody, Unconjugated (bs-1185R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
Blank control:Jurkat cells(blue). Primary Antibody:Rabbit Anti-TRAF3 antibody(bs-1185R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice. Primary antibody (bs-1185R,1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
