| 產(chǎn)品編號 | bsm-51265M |
| 英文名稱 | PINK1 Mouse mAb |
| 中文名稱 | 絲氨酸/蘇氨酸蛋白激酶PINK1單克隆抗體 |
| 別 名 | PINK1_HUMAN; PTEN Induced Putative Kinase 1; PTEN-Induced Putative Kinase Protein 1; BRPK; Serine/Threonine-Protein Kinase PINK1, Mitochondrial; Parkinson Disease(Autosomal Recessive) 6; Protein Kinase BRPK; EC 2.7.11.1; PARK6; |
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Specific References (4) | bsm-51265M has been referenced in 4 publications.
[IF=6.208] Chuanling Wang. et al. Berberine Rescues D-Ribose-Induced Alzheimer‘s Pathology via Promoting Mitophagy. INT J MOL SCI. 2023 Jan;24(6):5896 ICC ; Mouse.
[IF=6.025] Xuliang Zhang. et al. PINK1/Parkin-mediated mitophagy mitigates T-2 toxin-induced nephrotoxicity. FOOD CHEM TOXICOL. 2022 Jun;164:113078 WB ; Mouse.
[IF=4.872] Yajing Wang. et al. Long-term exposure to the fluoride blocks the development of chondrocytes in the ducks: The molecular mechanism of fluoride regulating autophagy and apoptosis. Ecotox Environ Safe. 2021 Jul;217:112225 WB ; Duck.
[IF=4.081] Zhang, Kai. et al. Long-Term Copper Exposure Induced Excessive Autophagy of the Porcine Spleen. BIOL TRACE ELEM RES. 2022 Jul;:1-9 WB ; Pig.
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| 研究領(lǐng)域 | 信號轉(zhuǎn)導(dǎo) 激酶和磷酸酶 線粒體 |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | 5C6 |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:200-1000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 63 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 線粒體 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant human PINK1 protein |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a serine/threonine protein kinase that localizes to mitochondria. It is thought to protect cells from stress-induced mitochondrial dysfunction. Mutations in this gene cause one form of autosomal recessive early-onset Parkinson disease. [provided by RefSeq, Jul 2008] Function: Protects against mitochondrial dysfunction during cellular stress by phosphorylating mitochondrial proteins. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy) by mediating activation and translocation of PRKN . Targets PRKN to dysfunctional depolarized mitochondria through the phosphorylation of MFN2 . Activates PRKN in 2 steps: (1) by mediating phosphorylation at 'Ser-65' of PRKN and (2) mediating phosphorylation of ubiquitin, converting PRKN to its fully-active form . Required for ubiquinone reduction by mitochondrial complex I by mediating phosphorylation of complex I subunit NDUFA10 (By similarity). Subunit: Interacts with PRKN. Interacts with FBXO7. Forms a complex with PRKN and PARK7. Subcellular Location: Localizes mostly in mitochondrion and the 2 proteolytic processed fragments of 55 kDa and 48 kDa localize mainly in cytosol. Tissue Specificity: Highly expressed in heart, skeletal muscle and testis, and at lower levels in brain, placenta, liver, kidney, pancreas, prostate, ovary and small intestine. Present in the embryonic testis from an early stage of development. Post-translational modifications: Autophosphorylation at Ser-228 and Ser-402 is essential for Parkin/PRKN recruitment to depolarized mitochondria. Two shorter forms of 55 kDa and 48 kDa seem to be produced by proteolytic cleavage and localize mainly in cytosol. Similarity: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. SWISS: Q9BXM7 Gene ID: 65018 Database links: Entrez Gene: 65018 Human Entrez Gene: 68943 Mouse SwissProt: Q9BXM7 Human SwissProt: Q99MQ3 Mouse |
| 產(chǎn)品圖片 |
All lanes : Anti-Pink1(115-213) at 1:4000 dilution Lane 1:
A431 whole cell lysate Lane 2: Mouse brain lysate
Lysates/proteins at 20 μg per lane. Secondary Goat
Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000
dilution. Predicted band size : 63 kDa Blocking/Dilution
buffer: 5% NFDM/TBST.
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